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Destabilization of hen-egg lysozyme (HEL) facilitates presentation of the antigenic determinants of HEL. Two T-cell hybridoma clones specific for HEL98–116/I-Ed (a) and HEL8–35/I-Ad (b) were cultured with HEL derivatives in the presence of A20 cells. Culture supernatants were harvested at 24 hr, and the levels of interleukin-2 (IL-2) were measured according to growth of CTLL-2, determined by metabolizing MTT and measuring the absorbance at 570 nm (A570).
