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. 2001 Nov;104(3):259–268. doi: 10.1046/j.1365-2567.2001.01314.x

Figure 7.

Figure 7

Time courses of serum antibody levels against native hen-egg lysozyme (HEL) and denatured HEL. Three groups of five BALB/c mice were immunized subcutaneously with 1 µg/mouse of 6,127CM-HEL, native HEL, or 35–108CL-HEL in complete Freund's adjuvant (CFA) on day 0 and intraperitoneally with 1 µg/mouse of the same antigen in incomplete Freund's adjuvant (IFA) on day 35 (a). Independently, three groups of five BALB/c mice were immunized intraperitoneally with 0·5 µg/mouse (on days 0 and 21) and 5 µg/mouse (on days 53 and 87) of HEL derivatives in alum adjuvant (b). Native HEL (upper row) and denatured HEL (lower row) were coated onto enzyme-linked immunosorbent assay (ELISA) plates and IgG2a, IgG1, IgA and IgE antibody levels in diluted sera were evaluated. Dilution of serum in ELISA: IgG2a, 1/100 for (a), 1/50 for (b); IgG1, 1/500 for (a), 1/50 for (b); IgA, 1/50 for (b); IgE, 1/20 for (a), 1/50 for (b). The data are expressed as mean absorbance at 405 nm (A405) plus the standard error, of five mice.