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. 2001 Nov;104(3):269–277. doi: 10.1046/j.1365-2567.2001.01323.x

Figure 4.

Figure 4

Enhanced TCR ζ−chain expression in M. tuberculosis granuloma-associated CD4+ T lymphocytes induced by IL-2. GAL were freshly isolated from pulmonary lesions, as described in detail in the Materials and Methods section, cultured for 3 days in the presence or absence of IL-2 (1000 IU/ml), gated on CD4+ T cells and stained for TCR ζ chain expression by flow cytometry using two different mAb as indicated. Differences in mean fluorescence channel (m.f.c.) intensity. CD4 + T-cells correspond to effector cells described in Table 1. ss=side scatter.