Table 2.

T-cell receptor usage in CD8⁺ T-cell lines (2.2, 2.4, 2.5, 2.15, 2.22 and 2.35)

	TCR VA chains						TCR VB chains
VA/VB	2.2	2.4	2.5	2.15	2.22	2.35	2.2	2.4	2.5	2.15	2.22	2.35
1			X	X	X	X
2
3		X	X			X	X		X		X
4								X	X		X
5										X
6									X
7
8									X	X
9
10
11
12							X					X
13								X
14
15
16											X	X
17
18
19
20
21
22			X
23			X
24
25
26
27
28
29				X

^*Not determined.

CD8⁺ peripheral blood lymphocytes (PBL) were stimulated with interleukin-4 (IL-4)/granulocyte–macrophage colony-stimulating factor (GM-CSF)-driven dendritic cells (DCs) pulsed with peptide VLTDGNPPEV, followed by cloning of T-cell lines in 96-well plates at 1, 10, or 100 T cells/well (10 plates for each concentration). T cells were stimulated weekly with 3000 rads irradiated autologous peripheral blood mononuclear cells (PBMCs) pulsed with the peptide VLTDGNPPEV in AIM-V medium containing 50 IU/ml of IL-2 and 50 ng/ml of IL-7. T cells that could be expanded were seeded in 24-well plates. RNA was isolated from each cell line and reverse transcribed into cDNA. Each T-cell receptor (TCR) variable alpha chain (VA) or variable beta chain (VB) expression was tested by reverse transcription–polymerase chain reaction (RT–PCR) analysis. Owing to the low number of cells available from T-cell line 2.2, examination of the TCR VA chains was not possible. The T-cell lines were from individual 2.