Table 3.
T-cell receptor (TCR) usage in clonal/oligoclonal T cells defining a human leucocyte antigen (HLA)-A2-presented peptide from Mycobacterium tuberculosis
| T-cell clones/lines | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| TCR | TCR VA/VB | CDR3 | Joining region | 2.2* | 2.4 | 2.5 | 2.15 | 2.22 | 2.35 | |
| VA1S1 | AEYFCA | VKP | GGGADGLTFGKGTJLII | AJ45 | X | X | X | X | ||
| VA3 | SYFCA | TVLSF | YNQGGKLIFGQGTE | AJ23 | X | X | ||||
| VA3 | SYFCA | TS | GGSYIPTFGRGTSL | AJ6 | X | |||||
| VA22 | QVSDSAVYFCA | LG | TGGGNKLTFGTGTQLKVN | AJ10 | X | |||||
| VA23 | QPGDSATYLCA | VSPL | NSGNTPLVFGKGT | AJ29 | X | |||||
| VA29 | QLSYSGTYFCG | TTI | GGSQGNLIFGKGTKLSVKP | AJ42 | X | |||||
| VB3 | TNQTSMYLCA | SSLQR | TEAFFGQGT | BJ1.1 | X | X | X | |||
| VB4 | PEDSSIYLCS | VGD | YGYTFGSGTRLTV | BJ1.2 | X | X | X | |||
| VB5 | ELDDSALYLCA | WGTPELP | YEQYFGPGTRLT | BJ2.7 | X | |||||
| VB6S7 | TQQEDSAVYLCA | SSNRDSPL | GELFFGEG | BJ2.2 | X | |||||
| VB8 | IQPSEPRDSYVYFCA | SSLYSGGRE | EQFFGPGTRLT | BJ2.7 | X | X | ||||
| VB12 | SSGTSVYFCA | ISESRNG | YNEQFFGPGH | BJ2.1 | X | X | ||||
| VB13S6 | LRELAAPSQTSVYFCA | SSYLPGTGETS | QHFGDGTR | BJ1.5 | X | |||||
| VB16 | LEDSGVYFCA | SSQGSG | NEQFFGPG | BJ2.1 | X | |||||
| VB16 | LEDSGVYFCA | SSQLSGQV | NEKLFFGSGTQLS | BJ1.4 | X | |||||
| GM-CSF | GM-CSF | GM-CSF | GM-CSF | GM-CSF | GM-CSF | |||||
| IL-4 | IL-4 | IL-4 | IL-4 | |||||||
Not determined for VA expression.
T cells were cloned using repetitive stimulation with the peptide VLTDGNPPEV and seeded in 96-well plates at 1, 10, or 100 cells/well. After restimulation using autologous PBMCs plus peptide, T cells were expanded and tested for T-cell receptor (TCR) variable alpha chain (VA) of variable beta chain (VB) expression. Each TCR VA/VB chain was directly sequenced. Note that some CD8+ T-cell lines utilize the identical TCR, other use the same variable region, but a different complementarity-determining region 3 (CDR3). The CDR3 region determines the antigen specificity. The cytokine-secretion pattern of these CD8+ monoclonal or oligoclonal T-cell lines is compiled as defined by interleukin-4 (IL-4) or granulocyte–macrophage colony-stimulating factor (GM-CSF) release. No interferon-γ (IFN-γ) secretion could be detected. This compilation refers to Fig. 2. Shared usage of aa within the highly polymorphic CDR3 region is indicated in bold.