Table 4.

Cytokine-secretion pattern in Mycobacterium tuberculosis 19-kDa peptide-sorted T cells

	Patient 1		Patient 2		Patient 3
Targets	IFN-γ	IL-4	IFN-γ	IL-4	IFN-γ	IL-4
T2-cells loaded with peptides from:
M. tub. 19 kDa	0	60	0	64	108	52
gp100	0	0	0	0	20	0
Diluents	0	0	0	0	0	0

Cytokine release is expressed as pg/ml/24 hr.

M. tuberculosis 19-kDa peptide (VLTDGNPPEV)-specific T cells were isolated from freshly harvested peripheral blood lymphocytes (PBL) from human leucocyte antigen (HLA)-A2⁺ patients with active pulmonary tuberculosis and tested for target recognition using the nominal T-cell epitope used for the tetramer-sorting procedure. T2 cells with diluent alone or pulsed with a control peptide from the melanoma-associated antigen gp100 served as controls. M. tuberculosis 19-kDa tetramer-sorted T cells from three of three patients secreted interleukin-4 (IL-4) and T cells from one of three patients secreted interferon-γ (IFN-γ).