Table 1.
C3-fragment deposition and MAC formation on B cells following incubation with various sera
| Serum | C3-fragment deposition* | % of autologous | C9 deposition* | % of autologous | Ratio‡ |
|---|---|---|---|---|---|
| Autologous† | 142 | 100 | 43·3 | 100 | 1:1 |
| Donor 1 | 71·6 | 50·4 | 20·1 | 46·4 | 1:0·92 |
| Donor 2 | 83·6 | 58·9 | 17·2 | 39·7 | 1:0·67 |
| Donor 3 | 78·1 | 55·0 | 18·1 | 41·8 | 1:0·76 |
| C8 deficient† | 28·0 | 19·7 | 0·2 | 0·5 | 1:0·025 |
*
Difference in values (mean fluoresence intensity units, MFI) for cells incubated in normal human serum (NHS) or NHS/EDTA, upon probing with FITC-conjugated anti-C3d and anti-C9 mAb, respectively.
†
Mean values from two experiments.
‡
Ratio of relative efficiency for C3-fragment:C9 deposition.