Figure 3.
Effect of tumour necrosis factor-α (TNF-α) neutralization on the macrophage (Mφ) nitrite response to lipopolysaccharide (LPS). Murine peritoneal Mφs were cultured in medium alone, or with LPS (10 µg/ml) in the presence or absence of the neutralizing anti-TNF-α antibody TN3.19.12. After 48 hr, culture supernatants were harvested and nitrite levels determined by the Griess reaction. Results are expressed as mean±SEM and are representative of two similar experiments. *P < 0·01, TN3.19.12 treatment compared to the LPS-alone control.