Fig. 4.
Role of the Tat transport system in extracellular signal production. The ability of CM from the indicated strains to activate the cma37::lacZ fusion in XD37 is shown. (A) The ability of a TatAPs protein missing amino acids 2–8 to restore signal production is shown. (B) The production of signal from tatC and aarA mutants is shown. For these experiments, CM was harvested at an OD of 0.9. The values represent fold-activation relative to the same cells grown in control LB and were determined from quadruplicate samples from two independent experiments.