Table 2.
The two-allele mutant set
| n | |
|---|---|
| Mutants included | 3,050 |
| Gene representation | |
| Genes represented | 1,448 |
| Genes with 1 internal insertion | 203 |
| Genes with 2 internal insertions | 1,014 |
| Genes with 3 internal insertions | 193 |
| Genes with 4 internal insertions | 29 |
| Genes with 5 internal insertions | 9 |
| Insertions outside of gene ORFs* | 79 |
| Genes not represented | 319 |
| Transposon types | |
| <KAN-2 > insertions | 366 |
| T15 insertions | 3 |
| T17 insertions | 26 |
| T18 insertions | 487 |
| T20 insertions | 2,168 |
| Re-sequence-mapping | |
| Insertion locations confirmed | 2,785 |
| Insertion locations unconfirmed† | 265 |
All mutants were single-colony purified and resequence-mapped to verify their identities.
*Some insertions outside of predicted genes were included for (i) insertions in promoter regions of genes lacking coding sequence insertions and (ii) insertions in long intergenic regions.
†Sequence-mapping quality was insufficient to identify the transposon insertion site. Based on the fraction of sequence confirmed strains that were of expected identity, we estimate that 92% of these strains are correctly identified.