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. 2007 Jan 9;104(3):1045–1050. doi: 10.1073/pnas.0610216104

Fig. 5.

Fig. 5.

Decrease in intracellular ROS production by UCP-1 overexpression or by NAC-mediated scavenging attenuates drug-mediated increase of CYP2H1, CYP3A37 and ALAS1 gene expression and AMPK activity. (A) LMH cells transiently transfected with UCP-1 were incubated with 500 μM PB or metyrapone, or 1 mM AICAR for 16 h. Gene expression was detected by RT-PCR. ∗, P < 0.01. (B) LMH cells were incubated with 500 μM PB or metyrapone, or 1 mM AICAR, with or without 10 mM NAC for 16 h. mRNA levels were measured by RT-PCR. ∗, P < 0.01. (C) LMH WT or LMHρ0 cells were incubated with 500 μM PB or metyrapone for 16 h. mRNA levels were detected by RT-PCR. ∗, P < 0.01. (D) Activation of AMPK after 1 h treatment with 500 μM PB or metyrapone, or 1 mM AICAR is shown by Western blot evidencing the phosphorylation of AMPK-Thr-172 and ACC-Ser-79. M, metyrapone, AIC, AICAR.