In situ RNA hybridization of B class genes in Streptochaeta, Elegia, and Joinvillea. (A) SaAP3 is strongly expressed in the developing stamens (St) and the whorl containing bracts X–XII. Weaker expression is evident in the carpel and possibly the VII–VIII whorl. (B) SaPI2 is expressed strongly in the stamen whorl and the X–XII whorl, as is SaAP3. (C) EeAP3a is strongly expressed in the emerging stamens and inner tepals (IT) but absent from the outer tepals (OT). (D) EeAP3b expression is very similar to EeAP3a: strong in stamens and inner tepals but absent from outer tepals. (E) JaAP3 expression can be seen in the stamens and inner tepal, but the adjacent outer tepal is relatively lacking in JoinAP3 expression. (F) JaPI expression, like JaAP3, is seen in the stamens and inner tepals but relatively absent from the outer tepals. (Scale bars: 100 μm.)