Fig. 4.

AICAR protects calcium depletion-induced tight junction disassembly. MDCK cells were grown on Transwell filters (A) or glass coverslips (B) in normal growth medium for 3 days and changed to low calcium medium (LCM) in the presence or absence of 2 mM AICAR. (A) TER was measured at the indicated time points and normalized to the baseline values before switching to low calcium medium. (B) Cells were fixed at 1 h after being changed to low calcium medium and stained with the indicated antibodies.