Fig. 2.

2-DG decreases CtBP recruitment to the SIRT1 promoter and increases SIRT1 expression. (A) CtBP recruitment to the SIRT1 promoter is blocked by 2-DG. Human primary fibroblasts were treated with 2-DG for 3 h, and ChIP was performed with antibodies directed against CtBP. Recruitment of CtBP to the HIC1-binding sites on the SIRT1 promoter was assayed by PCR. LINE-1 served as nonspecific control. (B) 2-DG increases SIRT1 transcript levels. Primary human fibroblasts were treated with 2-DG for 6 or 9 h, and the SIRT1 transcript was measured by RT-PCR. Data were normalized to 18S RNA and are represented as mean ± SEM from three independent experiments. (C) 2-DG increases SIRT1 protein levels. Primary human fibroblasts were treated with 2-DG for 6 or 9 h, and SIRT1 protein was assayed by Western blotting. Tubulin served as a loading control.