Table 1.

PCR primers

Antigen	Forward primers	Reverse primers	Product length, bp
HPA-1	5′ss-HPA-1F	5′bio-HPA-1R	224
	(5′ ss-TTCTGATTGCTGGACTTCTC)	(5′bio-GGAGTGCAATCCTCTGGG)
HPA-2	5′bio-HPA-2F	5′ss-HPA-2R	179
(ref. 16)	(5′bio-GCCTGCTCTCACCGTCCT)	(5′-ss-AGTCAAGTTGTTGTTAGCCA)
HPA-4	5′bio-US-1	5′ss-US-2
	(5′-bio-GCC AGG GTT TTC CCA GTC AC)	(5′ss-CGA ATT CGA GCT CGG TAC CC)
	US-1-HPA-4F	US-2-HPA-4R	270
	(5′-GCC AGG GTT TTC CCA GTC ACC	(5′-CGA ATT CGA GCT CGG TAC CCA
	GGC AGG TGG AGG ATT AC)	CTT ACT CAT AGC AGG GGT TTT)

bio, biotin; 5′ss, 5′-HO-(CH₂)₆-S-S-(CH₂)₆)-.