Developing cf45-1− cells move more slowly than parental Ax2 cells. Cells were starved in submerged culture for 6 h, and then fields of cells were observed by time-lapse videomicroscopy. For each cell, the approximate center of the cell was tracked for 5 min, and the cell speeds were then binned in 1 μm/min increments. The average speeds were 3.3 ± 0.2 μm/min for Ax2 cells and 1.9 ± 0.1 μm/min for cf45-1− cells (means ± standard errors of the means for 60 cells each, combining the observations from 20 cells in three separate experiments). A two-tailed Mann-Whitney test showed that the speed distributions were different, with a P value of <0.005.