Skip to main content
. 2003 Aug;2(4):788–797. doi: 10.1128/EC.2.4.788-797.2003

FIG. 3.

FIG. 3.

Disruption of cf45-1 results in cells lacking the cf45-1 mRNA and the CF45-1 protein. (A) Northern blots of RNA from Ax2 parental and cf45-1− cells were probed with fragments of cf45-1 (top row), countin (middle row), or cf50 (bottom row). (B) Western blots of Ax2 parental (WT) or cf45-1− vegetative cells were stained with anti-CF45-1, anti-countin, or anti-CF50 antibodies; the position of molecular mass markers is shown at left. (C) A Western blot of 20-h CM (containing material secreted by developing cells) from the indicated cell lines was stained with anti-CF45-1 antibodies. (D) A Western blot of the indicated amounts of either recombinant CF45-1 or recombinant CF50 was stained with anti-CF45-1 antibodies; this indicates that the 50-kDa band that the anti-CF45-1 antibodies binds to is CF50. The molecular mass of the recombinant CF45-1 (including the His tag) is 31.5 kDa, and the mass of the recombinant CF50 is 33 kDa.