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CF45-1 appears to be part of a large complex in the CM from starving cells. CM was made from Ax2 parental or cf50− cells and then concentrated ∼10-fold. After size fractionation on a sieving gel column, the fractions were assayed for CF45-1 by Western blotting. Staining of the fractions from Ax2 CM was previously shown for countin and CF50; both proteins elute with a peak at fraction 37 (3). Molecular mass standards eluted with peaks at fractions 37 (443 kDa) and 41 (66 kDa).
