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Growing cells secrete CF45-1. The indicated cell types were inoculated into HL5 medium at 1 × 106 cells/ml and grown in shaking culture for 20 h. The conditioned growth medium was clarified by centrifugation. (A) A Western blot of the conditioned growth media from the indicated cell types was stained with anti-CF50 antibodies. Similar blots were stained with anti-CF45-1 (B) and anti-countin (C) antibodies.
