Fig. 5.

Inhibitors of calpain and the proteasome increase WASP levels in T cells from WIP^−/− mice. T cells from WT and WIP^−/− mice were incubated with medium containing DMSO vehicle (Med), MG132 (M), calpeptin (C), or both (M+C) for 6 h, then analyzed for WASP by flow cytometry by using mAb B-9 conjugated to phycoerythrin (A) or by Western blot using B-9 mAb (B). Expression of Vav1 was used as control. The numbers in B represent the ratio of WAS:Vav1 in inhibitor-treated cells versus untreated control cultured with medium alone.