FIG. 3.

Alignment of various fungal transporters and transporter-like proteins. (A) Alignment of deduced protein sequences, from top to bottom, for Gtt1 from T. harzianum, Hgt1 from K. lactis, HXT1 from U. fabae, Snf3, Gal2, and Hxt2 from S. cerevisiae, and Rco3 from N. crassa. Sequences were aligned by using the CLUSTAL W algorithm (17). Horizontal black bars indicate the transmembrane domains of Gtt1 obtained by using the TopPred algorithm (54). Black shading indicates highly conserved residues, and grey shading indicates lesser degrees of similarity. The black arrow indicates Arg151, the striped arrow indicates Phe412, and the grey arrow indicates Trp425 (positions relative to those of the Gtt1 sequence).