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Relative in vitro PKA activities measured in protein extracts prepared from N. crassa wild-type and cot-1 cultures 5 h after a shift from permissive (25°C) to restrictive (37°C) growth conditions. Cultures were grown in the presence of NaCl (1.0 M), sorbitol (1.25 M), ethanol (EtOH) (7.5%), or H2O2 (10 mM). NT, not treated. The nonphosphorylated and phosphorylated (indicating activity) fluorescent Kemptide substrates migrated to the anode and cathode of the agarose gel, respectively.
