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. 2007 Jan 4;26(2):301–312. doi: 10.1038/sj.emboj.7601498

Figure 5.

Figure 5

(A) Localization of different endocytic compartmental markers in wild-type and sand-1(or552) mutant coelomocytes. Worms were grown to young adults at 20°C and shifted to 25°C 4–5 h before the experiment. RME-1::GFP marks recycling endosomes, RME-8::GFP is associated with early and late endosomes, and CUP-5::GFP and LMP-1::GFP staining represent mostly lyosomes. Localization of GFP::SAND-1 in wild-type coelomocytes. The bar represents 5 μm. (B) Quantification of the size of endosomes and lysosomes in wild-type and sand-1(or552) coelomocytes. Organelles, which were positive for the indicated marker, were sorted into four size categories according to their diameter: 1–2, 2–4, 4–6, and 6–10 μm. Error bars represent the standard deviation.