Skip to main content
. 2006 Dec 21;26(2):459–467. doi: 10.1038/sj.emboj.7601494

Figure 3.

Figure 3

Poliovirus translation in HeLa cell cytoplasmic extracts that lack SRp20. (A) SRp20 in poly(rC)-depleted HeLa S10 extracts. A Western blot using a monoclonal antibody to SRp20 shows the analysis of different HeLa S10 extracts that were either mock-depleted (lanes 1 and 3) or poly(rC) depleted (lanes 2 and 4). S10 #1 and S10 #2 were derived from two different cytoplasmic extracts prepared and depleted independently. Protein size markers from the SDS–polyacrylamide gel used to generate this blot are indicated to the right of panel. (B) Poliovirus translation in HeLa S10 extracts that lack PCBP and SRp20. In vitro translation assays in HeLa cytoplasmic S10 extracts were carried out using luciferase reporter RNAs preceded by the poliovirus 5′ NCR. Increasing amounts of recombinant PCBP2 were added to HeLa extracts that were depleted of PCBP but contained SRp20 (cross-hatched bars, S10 #2) or to extracts that were depleted of both PCBP and SRp20 (black bars, S10 #1). Translation activity is depicted as relative light units of firefly luciferase.