Figure 5.

Downregulation of SRp20 decreases poliovirus translation in HeLa cell lysates. In vitro translation assays were carried out in HeLa cytoplasmic S10 extracts using a poliovirus 5′ NCR luciferase construct (5′PVLuc). Increasing amounts of IgG from a mouse monoclonal antibody to SRp20 were added to translation reactions (black bars). Normal mouse IgG was added as a negative control to examine the effect of nonspecific IgG on translation (gray bars). Translations without IgG added were set as 100% (open bar). Translation activity is depicted as relative light units of firefly luciferase.