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. 2007 Jan 24;26(2):424–435. doi: 10.1038/sj.emboj.7601517

Figure 5.

Figure 5

(A) Knock-down of HP1γ results in increased virus production in U1 cells, a cellular model for HIV-1 latency. U1 cells were transfected with siRNA as indicated in the figure. Efficiency of HP1α, HP1β and HP1γ knock-down was analyzed 48 h after transfection by Western blotting using a specific antibody. Virus production was monitored 48 h post-transfection by measuring p24 antigen in culture supernatant. Cells were treated with TNFα for 12 h when indicated. (B–D) HP1γ-mediated inhibition of HIV-1 replication is established early after virus infection. Jurkat cells were transfected with either control siRNA or HP1γ-, p300- or HP1α-specific siRNA. At 48 h post-transfection, cells were either (B) analyzed for expression levels of HP1γ, HP1α and p300 or (C) infected with HIV-1, and virus replication was monitored every 3 days after infection by measuring p24 viral antigen in culture supernatant or (D) infected with a single-round infectious virus (HIV-1VSV-luc) and virus production was monitored 48 h after infection by measuring luciferase activity in cell extracts.