Table 1.

Mutations in mef-2 and hda-4 suppress the reduced CR gene expression phenotype of kin-29 mutants

Straina	% expressing str-1∷gfp at WT levels	n	P-values
Wild type	100	280
kin-29(oy39)	0	218	<0.001
kin-29(oy38)	0	267	<0.001
mef-2(gv1)	100	155
mef-2(oy65)	100	223
hda-4(oy57)	100	189
mef-2(oy63); kin-29(oy39)	100	204
mef-2(oy65); kin-29(oy39)	100	158
mef-2(oy65); kin-29(oy38)	100	188
mef-2(gv1); kin-29(oy39)	100	209
mef-2(gv1); kin-29(oy38)	100	194
mef-2(gv1); kin-29(oy39); Ex[gfp-tagged mef-2 genomic]	5	121	<0.001
mef-2(oy65); kin-29(oy39); Ex[srd-23∷mef-2]	25	153	<0.001
mef-2(oy65); kin-29(oy39); Ex[lim-4∷mef-2]	27	174	<0.001
kin-29(oy38) hda-4(oy57)	100	201
kin-29(oy39) hda-4(oy59)	100	211
kin-29(oy38) hda-4(ok518)	0	224	<0.001
kin-29(oy39) hda-4(ok518)	0	228	<0.001
kin-29(oy38) hda-4(oy57)/ hda-4(ok518)	96	144
rrf-3(pk1426); kin-29(oy38) hda-4(RNAi)b	21	65	<0.001c
kin-29(oy38) hda-4(oy57); Ex[gfp-tagged hda-4 genomic]	11	155	<0.001
kin-29(oy38) hda-4(oy57); Ex[odr-4∷hda-4∷gfp]	8	146	<0.001
kin-29(oy38) hda-4(oy57); Ex[lim-4∷hda-4]	10	188	<0.001
Straina	% expressing sra-6∷gfp at WT levels	n	P-values
Wild type	100	234
kin-29(oy38)	0	245	<0.001
mef-2(gv1)	100	153
hda-4(oy57)	100	101
mef-2(gv1); kin-29(oy38)	100	167
kin-29(oy38) hda-4(oy57)	100	189
Adult animals grown at 20°C were examined. Shown are the percentages of animals expressing str-1∷gfp or sra-6∷gfp in at least one AWB or ASH neuron, respectively, at wild-type levels (× 50 magnification). P-values were determined using a two-sample t-test between proportions. Only significant differences (P<0.001), compared to wild type unless indicated otherwise, are shown.
aFor strains carrying extrachromosomal arrays, data shown are the averages of two or more independent transgenic lines. All strains carry integrated copies of str-1∷gfp or sra-6∷gfp.
bAnimals were fed with bacteria expressing hda-4 dsRNA, starting at the L4 larval stage, and str-1∷gfp expression was examined 24 h later.
cData compared to kin-29(oy38).