Table 2.
The HDA-4(S198A) mutation constitutively represses str-1∷gfp expression
| Straina,b | % expressing str-1∷gfp at WT levels | n | P-values |
|---|---|---|---|
| Wild type | 100 | 120 | |
| kin-29(oy38) | 0 | 120 | <0.001 |
| hda-4(oy57) | 100 | 109 | |
| Ex[hda-4∷hda-4] | 100 | 110 | |
| Ex[hda-4∷hda-4 (S198A)] | 18 | 112 | <0.001 |
| kin-29(oy38); Ex[hda-4∷hda-4] | 0 | 102 | <0.001 |
| kin-29(oy38); Ex[hda-4∷hda-4 (S198A)] | 0 | 105 | <0.001 |
| hda-4(oy57); Ex[hda-4∷hda-4] | 100 | 117 | |
| hda-4(oy57); Ex[hda-4∷hda-4 (S198A)] | 17 | 115 | <0.001 |
| kin-29(oy38) hda-4(oy57) | 100 | 119 | |
| kin-29(oy38) hda-4(oy57); Ex[hda-4∷hda-4] | 10 | 108 | <0.001 |
| kin-29(oy38) hda-4(oy57); Ex[hda-4∷hda-4 (S198A)] | 11 | 110 | <0.001 |
| Adult animals grown at 20°C were examined. Shown are the percentages of animals expressing str-1∷gfp in at least one AWB neuron at wild-type levels (× 50 magnification). P-values were determined using a two-sample t-test between proportions. Only significant differences (P<0.001), compared to wild type, are shown. | |||
| aAll strains contain stably integrated str-1∷gfp fusion genes. | |||
| bFor strains carrying extrachromosomal arrays, data shown are the averages of two or more independent transgenic lines. All hda-4 genomic sequences are tagged with gfp coding sequences. | |||