Table 4.
lf mutations in calcineurin suppress kin-29(lf) mutations
| Straina,b | % expressing str-1∷gfp at WT levels | n | P-values |
|---|---|---|---|
| Wild type | 100 | 250 | |
| kin-29(oy39) | 0 | 250 | <0.001 |
| kin-29(oy38) | 0 | 250 | <0.001 |
| Calcineurin | |||
| cnb-1(jh103) | 100 | 206 | |
| tax-6(p675) | 100 | 233 | |
| cnb-1(jh103); kin-29(oy39) | 27 | 180 | <0.001c |
| cnb-1(jh103); kin-29(oy38) | 36 | 145 | <0.001c |
| tax-6(p675); kin-29(oy39) | 32 | 155 | <0.001c |
| SUMO/CDK5 | |||
| smo-1(ok359) | 100 | 102 | |
| cdk-5(ok626) | 100 | 155 | |
| smo-1(ok359); kin-29(oy39) | 0 | 108 | c |
| cdk-5(ok626); kin-29(oy39) | 0 | 167 | c |
| mef-2(oy65); kin-29(oy39) | 100 | 240 | |
| mef-2(oy65); kin-29(oy39); Ex[mef-2∷mef-2 genomic] | 9 | 195 | <0.001 |
| mef-2(oy65); kin-29(oy39); Ex[mef-2∷mef-2 (K316R)] | 6 | 164 | <0.001 |
| mef-2(oy65); kin-29(oy39); Ex[mef-2∷mef-2 (S321A)] | 6 | 200 | <0.001 |
| Ex[mef-2∷mef-2 genomic] | 100 | 145 | |
| Ex[mef-2∷mef-2 (K316R)] | 100 | 134 | |
| Adult animals grown at 20°C were examined. P-values were determined using a two-sample t-test between proportions. Only significant differences (P<0.001), compared to wild type or kin-29 as indicated, are shown. | |||
| aAll strains contain stably integrated str-1∷gfp fusion genes. | |||
| bFor strains carrying extrachromosomal arrays, data shown are the average of three or more independent transgenic lines. | |||
| cData compared to kin-29(oy38) and kin-29(oy39). | |||