Fig. 3.

Northern blot analysis of total RNA from individual G₁ and G₂ hens. (A–D) Total RNA was extracted from the magnum portion of the oviduct (M), spleen (S), pancreas (P), brain (B), intestine (I), liver (L), and breast muscle (Br) from: two G₁ hens (OR2-4:447 and OR2-4:476, A) and two G₂ hens (OR2-4:25:59 and OR2-4:25:171, B) carrying the OVA-miR24 vector; two G₂ hens carrying the EREOVA-IFN vector (EOI3-13:148:14 and EOI3-13:148:40, C), and a nontransgenic control hen (D). Blots were sequentially hybridized with probes for either miR24 (R) or hIFNβ1a (I), followed by an ovalbumin probe (O) and then 18S ribosomal RNA (18S), with the exception of the control, which was hybridized sequentially with probes for miR24, hIFNβ1a, ovalbumin, and 18S ribosomal RNA.