Figure 3.

Restoring rut in a subset of MB α/β neurons results in partial rescue of the learning-deficiency of rut mutants for two odor pairs. Three-minute memory rescue in lines carrying GAL4 drivers that express in α/β neurons. (A,B,C) Rut rescue with c739-GAL4 using different odor combinations. Flies bearing c739-GAL4 in combination with the UAS-rut transgene demonstrated no significant rescue of rut memory defect over the mutant control group rut²⁰⁸⁰; c739-GAL4 for odor combinations MCH-BEN (P = 0.0886, n = 12), MCH-OCT (P = 0.7554, n = 10), and OCT-BEN (P = 0.6218, n = 12). For all odor combinations tested, rut²⁰⁸⁰; c739-GAL4; UAS-rut flies performed significantly differently from control flies that were CS; c739-GAL4 (P < 0.0001), but were indistinguishable from control flies that were rut²⁰⁸⁰; c739-GAL4. (D–F) Rut rescue with 17d-GAL4, a line that expresses in a subset of α/β neurons, using different odor combinations. Flies bearing the GAL4 driver 17d in combination with the UAS-rut transgene demonstrated significant rescue of the rut memory defect over the mutant control group rut²⁰⁸⁰; 17d-GAL4 for odor combinations MCH-BEN (P < 0.0001, n = 24) and OCT-BEN (P = 0.0047, n = 12), but not MCH-OCT (P = 0.2577, n = 12). For all odor combinations tested, rut²⁰⁸⁰; 17d-GAL4; UAS-rut flies performed significantly more poorly than control flies that were CS; 17d-GAL4 (P < 0.0001). In all experiments, the P.I.’s were subjected to a one-way ANOVA with genotype as the main effect, followed by post hoc analysis with the Bonferroni/Dunn test. Statistical significance (**) or nonsignificance (N.S.) is indicated.