Figure 4.

Odor-pair-specific disruption of 3-min memory when MB signaling from α/β neurons is blocked. Three-minute memory performance of flies carrying UAS-shi^ts1 in combination with the GAL4 drivers c739 or 17d with training and testing performed under permissive (25°C) and restrictive (32°C) conditions. The different odor combinations used for the experiments are indicated above the graphs: MCH-BEN (A,D), MCH-OCT (B,E), and OCT-BEN (C,F). (A,B,C) Performance of flies of the genotype c739-GAL4; UAS-shi^ts1 were indistinguishable from controls w; c739-GAL4 and w; UAS-shi^ts1 at the permissive temperature for MCH-BEN (P ≥ 0.2820, n = 6), MCH-OCT (P ≥ 0.4691, n = 10), and OCT-BEN (P ≥ 0.2569, n = 6), but showed a significant disruption of memory at the restrictive temperature for MCH-BEN (P ≤ 0.0127, n = 6), MCH-OCT (P ≤ 0.0014, n = 6), and OCT-BEN (P ≤ 0.0002, n = 9). (D–F) Performance of flies of the genotype 17d-GAL4; UAS-shi^ts1 were indistinguishable from controls w; 17d-GAL4 and w; UAS-shi^ts1 at the permissive temperature for MCH-BEN (P ≥ 0.0981, n = 6), MCH-OCT (P ≥ 0.7679, n = 10), and OCT-BEN (P ≥ 0.0404, n = 13). Significant disruptions of memory were observed for 17d-GAL4; UAS-shi^ts1 flies at the restrictive temperature when compared to controls for MCH-BEN (P ≤ 0.0008) and OCT-BEN (P ≤ 0.0111) but not for MCH-OCT (P ≥ 0.2310, n = 10 for all). In all experiments, the P.I.’s were subjected to a one-way ANOVA with genotype as the main effect, followed by post hoc analysis with the Bonferroni/Dunn test. Comparisons among the three different genotypes were performed, producing three pairwise planned comparisons per experiment at each of the temperatures. Comparisons were not significant unless indicated by asterisks (**).