Table 2.

Odor and shock avoidance scores for the different fly stocks used in the shi^ts1 experiments

Odor avoidance was assayed by measuring the choice of naive flies between odor and fresh air at odor concentrations used during training and testing. Air was bubbled through an odorant:mineral oil mixture. For each odorant, a one-way ANOVA with genotype as the main effect was performed for groups tested under similar conditions (25°C or 32°C; n ≥ 6 per group). Post hoc analysis using the Bonferroni/Dunn test indicated that MCH odor avoidance of flies carrying the UAS-shi^ts1 transgene with GAL4 driver c739 was lower than the control line w; c739-GAL4 for both temperatures tested (P ≤ 0.0166). However, MCH odor avoidance of these flies was not significantly different from the avoidance of w; UAS-shi^ts1, another control strain used in our experiments (P ≥ 0.5653). No other significant differences were found in odor avoidance (P ≥ 0.0266). Electric shock avoidance was assayed by measuring the avoidance response of naive flies to 90 V DC shock. A one-way ANOVA with genotype as the main effect followed by post hoc analysis using the Bonferroni/Dunn test was performed for groups tested under similar conditions (25°C or 32°C; n = 6 per group). No significant differences for shock avoidance were observed (P ≥ 0.0459).