FIG. 1.
Analysis of IPLs in myoblasts infected with T. cruzi trypomastigotes. (A) Myoblasts (107) were labeled with [3H]inositol for 24 h and infected with 5 × 108 trypomastigotes for 3 h. Trypomastigotes were then washed out, and the myoblasts were incubated for different times in fresh medium (18, 24, and 48 h in lanes 2, 4, and 6, respectively). Control noninfected cells were prepared in the same way (lanes 1, 3, and 5). (B) Myoblasts labeled with [3H]palmitic acid and infected as described for panel A were incubated for 24 h (lane 2). A noninfected control is shown in lane 1. After incubation the radiolabeled lipids were extracted and analyzed by TLC with solvent system A. The positions of standards are indicated on the left. PIP2, phosphatidylinositolbisphosphate; I, inositol; IPn, IPs.