FIG. 2.

Analysis of IPLs in differentiating trypomastigotes in vitro. (A and B) T. cruzi trypomastigotes obtained from the culture medium of myoblasts were incubated with [³H]palmitic acid (A) at pH 7.5 for 2, 4, and 6 h (lanes 1, 3, and 5, respectively) or at pH 5 for the same times (lanes 2, 4, and 6, respectively) or with [³H]inositol (B) at pH 7.5 for 2, 4, and 6 h (lanes 7, 9, and 11, respectively) or at pH 5 for the same times (lanes 8, 10, and 12). (C) Trypomastigotes were incubated with [³H]inositol for 5 h. After that time, 10⁸ cells were extracted (lane 1) or were incubated for 5 additional h at either pH 7.5 (lane 2) or pH 5.0 (lane 3). The lipids were extracted and analyzed by TLC in solvent F. The arrow shows the main GIPL labeled with [³H]palmitic acid. c and d show bands corresponding to the lyso-PIs.