Figure 6. Heat-Shock Induction of mPer2 .

(A) Liver explants of LAP-tTA/TRE-Rev-erbα/mPer2::luc mice were cultured as in Figure 3 and subjected to heat shock (150 min at ∼40 °C) using homemade culture-dish heating devices, and luminescence was recorded as in Figure 3. Temperature plots are extrapolated from periodic temperature measurement. The time window during which organ cultures were exposed to an elevated temperature is depicted by a grey box.

(B) Lung explants were subjected to heat shock as in (A) at two different circadian times.