Figure 7.

Effects of IM7.8.1 antibody on the in vitro function of murine ECs. Studies were performed of the effects of anti-CD44 antibody (IM7.8.1) on various functions of the H5V murine EC line. A: The adhesion of the H5V cells to HA-coated surfaces was inhibited by IM7.8.1 (n = 4, *P < 0.001). B: The proliferation of H5V cells cultured for 24 hours in the presence of serum was assessed using a colorimetric assay and measurement of the reaction mixture at 490 nm. IM7.8.1 did not inhibit the proliferative response (n = 4). C: Linear defects were made in confluent cell monolayers, and closure of the wounds after 24 hours was assessed by computer-assisted image analysis (n = 6). Wound-induced migration was not inhibited by IM7.8.1. D: Apoptosis was assessed after 5 hours in the absence or presence of serum with IgG or IM7.8.1. IM7.8.1 did not induce apoptosis (n = 24). E: Shown are representative images of H5V cells plated on Matrigel that demonstrate that IM7.8.1 impairs tube formation.