Skip to main content
. Author manuscript; available in PMC: 2008 Jan 1.
Published in final edited form as: J Neurophysiol. 2006 Nov 8;97(1):837–848. doi: 10.1152/jn.00873.2006

Fig.2.

Fig.2

Adenosine decreases the frequency of sEPSCs in hypocretin/orexin neurons. Experiments were performed in the presence of bicuculline (30 μM) in all solutions and sEPSCs were recorded in hypocretin/orexin neurons held at −60 mV under voltage clamp. The frequency of sEPSCs decreased rapidly in the presence of adenosine (100 μM). Sample traces recorded in our experiments at various stages are presented in A and the time course of a typical experiment is plotted in B. Application of adenosine is indicated by the filled bar in B. C, pooled data from all tested hypocretin/orexin neurons at different concentrations of adenosine show that the inhibitory effect of adenosine on the frequency of sEPSCs is dose-dependent. The symbol “**” indicates P<0.01 (ANOVA test). D, frequency of sEPSCs obtained from all hypocretin/orexin neurons (n=8) examined in ACSF containing 2 mM glucose demonstrated that adenosine depressed the frequency of sEPSCs in hypocretin/orexin neurons (**, P<0.01, ANOVA). Inset, sample traces demonstrated that sEPSCs were completely blocked by ionotropic glutamate receptor antagonists AP5 and CNQX (n=7), suggesting a tone of glutamatergic transmission.