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. 2006 Dec;8(12):1011–1018. doi: 10.1593/neo.06610

Figure 2.

Figure 2

Properties of labeled phage. (A) Effect of increasing fluorochrome labeling (VT680) on phage affinity determined by ELISA (blue region: error range). (B) Effect of increasing labeling on phage fluorescence. Beyond 800 fluorochromes per M13 phage, there is little increase in fluorescence. (C) VT680-labeled phage clone (800 fluorochromes/phage) binds specifically to SPARC-expressing fibroblasts, but not to fibroblasts derived from SPARC-/- mice (scale bar: 10 µm). (D) Effect of successive phage labeling on viability (ability of the phage to infect and amplify in E. coli).