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. 2006 Dec;8(12):1062–1071. doi: 10.1593/neo.05832

Figure 3.

Figure 3

(A) Western blot analysis shows expression of UBE2C (∼ 19 kDa) in esophageal adenocarcinomas S96-T, M48-T, K32T, and P28-T. The Barrett's metaplasia sample M48-B and the normal esophageal sample M48-N express small amounts of UBE2C, whereas there was no UBE2C protein expressed in either the Barrett's dysplasia sample S96-BD or the normal esophageal samples A54-N and D67-N. (B) Western blot analysis of nine esophageal cell lines shows expression of UBE2C (∼ 19 kDa) in the esophageal adenocarcinoma cell lines BA, OE33, Flo-1, Seg-1, and Bic-1, whereas L20-T and H80-T showed smaller amounts of UBE2C protein. The squamous cell line Het-1A and the Barrett's metaplasia-derived S95-B also expressed UBE2C protein. β-Actin expression was used as loading control.