Figure 7.

c-Myc binds to the Bmi-1 promoter and regulates its activity. (A) c-Myc binds to the E-box sequences in the Bmi-1 promoter as shown by the ChIP analysis. The ChIP analysis was performed using vector control or Mel-18–overexpressing MCF10A and MCF7 cells as indicated. The cell lysates were IPed using c-Myc antibody or control IgG and a primer set that either amplifies the c-Myc binding flanking region in the Bmi-1 promoter (c-Myc site) or a region further upstream that does not contain a c-Myc binding site (Non-Myc site). (B) Detailed analysis of Bmi-1 promoter activity. The pGL-Bmi PrWT, pGL-Bmi PrMut, and pGL-Bmi PrΔMyc reporters (described in the text) were analyzed for the luciferase activity in 293T cells by transient transfection as described in Materials and Methods.