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Nuclear localization of the base, but not the lid, is affected by srp1-49. (A) ΔN rpn2 srp1-49 cells expressing Rpn7p-GFP or Rpn1p-GFP (YEK247 and YEK258, respectively) were cultured for 8 h at either 25 or 37°C, and localization of the GFP-fused components was observed under a confocal microscope. (B) Signals of A were quantified (fluorescence intensity per area) using the IPLab software, and ratio of the nuclear and cytosolic signals is shown. Error bars represent SD (n = 20).
