TABLE 2.
Mutation frequencies of the wild type and pos5 mutants
| Strain genotype | arg8m reversion ratea | Petite frequency (%)a,b | Nuclear mutation rate
|
|
|---|---|---|---|---|
| YPD | YPG | |||
| Wild type | 0.02 × 10−6 (1) | 4.9 (1) | 0.6 × 10−8 | 5.4 × 10−8 |
| pos5 | 1.00 × 10−6 (50) | 27 (6) | 0.25 × 10−8 | 6.4 × 10−8 |
| pFL39:mip1-exo− | 0.12 × 10−6 (6) | 20 (4) | NDc | ND |
| pos5/pFL39:mip1-exo− | ND | 72 (15) | ND | ND |
a
Reversion rate per cell per generation. Values in parentheses are normalized to the wild-type value.
b
Petite frequency is the combined frequencies of white versus total (red and white) colonies from three independent experiments done with the YPH925 strain with the pFL39 plasmid present.
c
ND, not done.