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. 2007 Feb;18(2):487–500. doi: 10.1091/mbc.E06-07-0592

Figure 1.

Figure 1.

ATPase activity and the C-tail region are essential for Drs2p function in protein trafficking. (A) Requirement for Drs2p ATPase activity and the C-tail region in Snc1p-GFP recycling. Plasmids pRS315 (empty), pRS315-DRS2 (DRS2), pDRS2-D560N (D560N, ATPase dead), and pRS315-Drs2-ΔCT (ΔCT) were introduced into strain ZHY615M2D (drs2Δ) along with pRS416-GFP-SNC1(GFP-Snc1). Transformants were grown at 30°C to mid-log phase and examined by fluorescence microscopy. Snc1-GFP is at the plasma membrane of DRS2 (wild-type) cells and is trapped in internal membranes in the drs2 mutants. (B) Requirement for Drs2p ATPase activity and the C-tail region in ALP transport to the vacuole. The same DRS2 plasmids as in A were cotransformed into strain ZHY2149D (drs2Δdnf1Δ) with pGO41 (GFP-ALP). Cells were grown at 30°C to mid-log phase, shifted to 15°C for 2 h, and then imaged. Fluorescent rings in the DRS2 (wild-type) cells are vacuoles, whereas GFP-ALP was mislocalized to extravacuolar puncta in drs2dnf1 mutants.