Figure 10.

GFP-Drs2p and GFP-Cdc50p do not accumulate in the prevacuolar compartment of class E vps mutants. vps27Δ and vps4Δ cells carrying pGFP-DRS2 and pRS425-CDC50 (A) or pGFP-CDC50 and pRS425-DRS2 (B) were grown to early mid-log phase at 30°C, labeled with 10 μg/ml FM4-64 on ice for 20 min, washed with fresh medium, and then chased at 30°C for 30 min before analysis. GFP and FM4-64 images were acquired separately and merged to show the coincidence of the two patterns. Arrowheads indicate the prevacuolar compartment.