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. 2007 Feb;18(2):487–500. doi: 10.1091/mbc.E06-07-0592

Figure 3.

Figure 3.

Mutation of Drs2p NPFXD motifs causes synthetic lethality with pan1-20. (A) Serial dilutions of ZHY823 (drs2Δ pan1-20 pRS416-DRS2) expressing the indicated constructs from LEU2-based plasmids were tested for growth at 30°C on medium with or without 5-FOA. Failure to grow on the +5-FOA medium indicates synthetic lethality between pan1-20 and the drs2 allele expressed from the LEU2 plasmid. (B) The experiment shown in Figure 2C was repeated to include ΔGIM combined with the NPW point mutations. (C) The drs2-npw1,2 allele is not synthetically lethal with arf1Δ. Serial dilutions of CCY2808 (arf1Δ drs2-2 pRS416-DRS2) expressing the indicated constructs from LEU2-based plasmids were tested for growth at 30°C on medium with or without 5-FOA.