Figure 7.

The pan1-20 mutant exhibits a constitutive defect in Ub-dependent endocytosis and a temperature conditional defect in NPFXD-dependent endocytosis. (A) Localization of GFP-Drs2p and GFP-Drs2-NPW1,2p in pan1-20 (TGY1906) cells. This pan1-20 strain expresses wild-type Drs2p from its endogenous locus to support viability. Cells were grown to mid-log phase at 27°C with or without shifting to 37°C for 1 h before imaging. Arrowheads indicate plasma membrane fluorescence. (B) Ste6p-GFP localization in wild-type and pan1-20 cells at 27°C. Wild-type (SEY6211) and pan1-20 (TGY1906) cells transformed with a plasmid harboring Ste6p-GFP (pSM1493) were grown at 27°C to mid-log phase and examined by fluorescence microscopy. Arrows indicate vacuoles. Arrowheads indicate plasma membrane fluorescence.