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. 2007 Feb;18(2):487–500. doi: 10.1091/mbc.E06-07-0592

Figure 8.

Figure 8.

Sequences in the Drs2p N-tail bearing PEST-like motifs mediate endocytosis redundantly with the NPFXD motifs. (A) The N-tail of Drs2p contains two potential PEST sequences and one poor PEST sequence. Arrowheads indicate the N-terminal truncation boundaries for the GFP-DRS2-ΔN2 and -ΔN3 alleles. (B) Localization of the GFP-Drs2p with N-terminal truncations in wild-type, sla1Δ, and pan1-20 cells. pGFP-DRS2, pGFP-Drs2-ΔN2, and pGFP-Drs2-ΔN3 were cotransformed with pRS425-CDC50 into wild-type (BY4742), sla1Δ (KLY011), or pan1-20 (TGY1907) cells. Transformants were grown to early log phase at 30°C and imaged by fluorescence microscopy at room temperature. Arrowheads indicate plasma membrane fluorescence. (C) Localization of the GFP-Drs2p with both the N-terminal truncation and the NPW1,2 mutations in wild-type cells. pGFP-Drs2-ΔN3-NPW1,2 was cotransformed with pRS425-CDC50 into wild-type cells. Transformants were grown to early log phase at 30°C and imaged by fluorescence microscopy at room temperature. Arrowheads indicate plasma membrane fluorescence.