Figure 3.
ER exit of misfolded proteins and subsequent vacuolar delivery and degradation depends on ER exit signal efficiency. (A) Wild-type (KHY298) cells expressing CFS (pAC812) or CFs′ (pAC815) were subjected to pulse-chase analysis as described. Graphic representations of the degradation of CFS and CFs′ are shown. (B and C) Wild-type (KHY298) and pep4Δ (KHY401) cells expressing CFS (pAC812) or CFs′ (pAC815) were subjected to pulse-chase analysis. Graphic representations of the degradation of CFS (B) and CFs′ (C) in wild-type and pep4Δ cells are shown.