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. 2007 Feb;18(2):455–463. doi: 10.1091/mbc.E06-08-0696

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© 2007 by The American Society for Cell Biology

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Figure 4. — CFs′ is an ERAD substrate and degraded by the proteasome. (A) Wild-type (KHY516) and cim5-1 (KHY517) cells expressing CFs′ (pAC815) were subjected to pulse-chase analysis as described. The degradation rate of CFs′ in wild-type and cim5-1 cells is shown. (B) Degradation of CFs′ was determined in wild-type (KHY298) and hrd1Δ (KHY299) cells. (C) Wild-type (KHY741) and rsp5-2 (KHY747) cells expressing CFs′ (pAC815) were grown at 24°C and shifted to the restrictive temperature (38°C) for 20 min before pulse-chase analysis. Graphic representations of the degradation of CFs′ in wild-type and rsp5-2 cells are shown.